Exposure of cell suspensions derived from kallus soy bean stalks to electric treatment or blended with plasmids Ri in the formation of kallus in multiple droplets

Exposure of cell suspensions derived from kallus soy bean stalks to electric treatment or blended with plasmids Ri in the formation of kallus in multiple droplets

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The present study successed in obtaining callus from embedding cell suspension of Glycine max in agar using Multiple Drop Array (MDA) technique.Results indicate that liquid Murashige sequal eclipse 5 battery and Skoog (MS) medium supplemented with 0.1 mg/L NAA and 2.0 mg/L BA promot divisions of cell and colonies formation.Moreover,electro-treatment of those cell suspension before culture enhanced division of cells and increased colonies formation.

Interestingly, the results indicate that mixing certain quantity of Ri-plasmid with cell suspension also stimulate formation of cellular colonies.Generally all these treatments led to the formation of small lock shock and barrel art pieces of callus in agar drops, These fragments of callus continued its growth on subculture medium.